Development of human-derived hemoglobin-albumin microspheres as oxygen carriers using Shirasu porous glass membrane emulsification

摘要

Novel hemoglobin-based artificial oxygen carriers are necessary in tissue engineering. We developed human hemoglobin (hHb) and albumin (HSA)-based microspheres using Shirasu porous glass (SPG) membrane emulsification. The obtained microspheres had a uniform size with an average diameter of 15.1 gm measured by optical microscope, which is similar to the diameter of human red blood cells (7-8 mu m). The loading amount of hHb in the microspheres was 20 wt%, which is similar to that of red blood cells (33 wt%). The hHb HSA microspheres showed similar oxygen dissociation behavior and methemoglobin formation resistance to native hHb. Incubation with genetically engineered HeLa cells that shows hypoxia-responsive EGFP expression demonstrated efficient oxygen supply from the microspheres. Our study suggests the utility of hHb HSA microspheres as oxygen carriers for tissue engineering with a low risk of infectious disease. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.