A case study in converting disposable process scouting devices into disposable bioreactors as a future bioprocessing tool

摘要

In this study, we perform mass transfer characterization (k _La) on a novel mechanically driven/stirred Process Scouting Device, PSD, (SuperSpinner D 1000?, SSD) and demonstrate that this novel device can be viewed as disposable bioreactor. Using patch-based optical sensors, we were able to monitor critical cell culture environmental conditions such as dissolved oxygen (DO) and pH in SSD for comparison to a 1L standard spinner (SS) flask. We also coupled these mass transfer studies with mixing time studies where we observed relative high mixing times (5.2min) that are typically observed in production scale bioreactors. Decreasing the mixing time 3.5-fold resulted in 30% increase in k La (from 2.3 to 3.0h ~(-1)) and minimum DO level increased from 0% to 20% for our model hybridoma cell line. Finally, maximum viable cell density and protein titer stayed within ±20% of historical data, from our standard 5L stirred bioreactor (Biostat?) operated under active DO control.