Batch Kinetics of Pseudomonas sp.Growth on Benzene.Modeling of Product and Substrate Inhibitions

摘要

Batch tests of benzene degradation were performed in liquid phase at 30 deg C pH 6.8 +- 0.2 and 200 rpm in two 3-L stirred tank bioreactors,using the benzene-degrading bacterium Pseudomonas sp.NCIMB 9688 A relatively high starting biomass level (220-270 mgx/L) and starting benzene concentration rangine from 20 to 200 mgs/L were selected as conditions to investigate possible inhibition phenomena.Volumetric as well as specific rates of biomass formation and substrate consumption were calculated from experimental data of both growth and benzene degradation and used to propose and check a new overall kinetic model for cell growth simultaneously accounting for both product and substrate inhibitions.The results of the present study evidenced the occurrence of a competitive-type product inhibition due to 2-hydroxy-muconic semialdehyde (K_ip'=0.902 mgs/L),which was stronger than the uncompeti-tive-type inhibition exerted by substrate (K_is=7.69 mgs/L).