Evaluation of IP10 and miRNA 269-a Expression Levels in Peripheral Blood Mononuclear Cell of Coronary Artery Disease Patients and Controls

摘要

Coronary artery disease (CAD) is the main cause of death worldwide. Atherosclerosis, the leading underlying cause of CAD, is a progressive inflammatory disease. miRNAs play a substantial role in inflammation. The aim of this study was to investigate the associations of peripheral blood mononuclear cells (PBMCs) gene expression of IP10 and miRNA 296-a and serum levels of IP10 and serum inflammatory cytokines interleukin-6 (IL-6) in CAD patients and controls. This is a case-control study conducted on 82 angiography confirmed CAD patients and 82 controls. PBMC expressions of miR-269a and IP10 were evaluated by real-time method, and serum concentrations of IL-6 and TNF-alpha were evaluated by enzyme-linked immunosorbent assay in the study population. A significant increase was found for serum IP10, IL-6, and TNF-alpha levels, and PBMC expression of IP10 and miRNA 296-a genes expression of CAD as comparison with controls. No significant correlation was found between IP10 gene expression and miRNA 296-a. A significant positive correlation was found between PBMC gene expression level of IP10 and serum concentrations of IP10 and cytokines IL-6 and TNF-alpha levels. Taking together, in PBMC of CAD patients, the IP10 and 296-a miRNA genes expression levels were increased significantly than controls. IP10, IL-6, and TNF-alpha levels in CAD patients were more than those in controls significantly. Concerning positive relationship between miRNA 269-a gene expression level and serum concentrations of IL-6 and TNF-alpha in CAD patients, it is proposed that IL-6 and TNF-alpha inhibitor could be the main targets of miRNA 296a and, thereby the IL-6 and TNF-alpha levels were increased; however, further study is needed.