i>miR-19-5p/i> Enhances Tumorigenesis in Human Colorectal Cancer Cells by Targeting i>TSPYL5/i>

摘要

The cancer suppressor gene, testis-specific protein Y-encoded-like 5 ( TSPYL5 ), has been implicated in various cancers, including colorectal cancer (CRC). In this study, we investigated the role of TSPYL5 in the development of CRC in vitro . First, we used bioinformatics to predict the binding target of TSPYL5 , and found that the microRNA, miR-19-5p , bound to the 3′ untranslated region (UTR) of TSPYL5 . This interaction was further validated by the dual-luciferase assay. Second, we found that overexpressed TSPYL5 enhanced apoptosis in HT29 cells and reduced cell proliferation, reduced cell migration/invasion, and most of the cells accumulated in the G0/G1 phase of the cell cycle. These effects were reversed after addition of miR-19-5p mimics. Third, knocking down expression of miR-19-5p also increased apoptosis, and reduced cell proliferation, migration, and invasion in HT29 cells. We speculate that miR-19-5p induces the degradation of TSPYL5 by binding to its 3′UTR. Our results suggest that increasing the expression of TSPYL5 in HT29 cells or inhibiting miR-19-5p promotes apoptosis of HT29 cells. Thus, miR-19-5p could be used as biomarkers of CRC, with potential implications for diagnosis and therapeutic intervention.