Simultaneous prediction of human antithrombin III and main metabolites inanimal cell culture processes by near-infrared spectroscopy

摘要

Samples of cell culture supernatants were analysed by near-infrared (NIR) spectroscopy. Spectral information was used to generate calibration models by using multivariate partial least squares (PLS) regression. Concentration predictions by these models were in good agreement with the values produced by conventional methods. Due to the simple pre-treatment of the sample and the short time required for measurement and prediction, NIR is a promising tool for automation of a cell culture process.