Broth Recycling To Reduce Process Noise Resulting from Concentrated Substrate Addition in Fed-Batch Cultivation of Escherichia coli

摘要

In this work feed hardware for fed-batch cultivation is presented (broth recycle feed injection system or BRFIS).BRFIS proved superior to conventional submerged or dripped feed systems in reducing dissolved oxygen (DO) oscillations during Escherichia doli fed-batch cultivation (5 min coefficient of variation of 0.7% for BRFIS as compared to 26% or greater for conventional feeding hardware in a 2 L test reacftor).Hence BRFIS is useful for fed-batch cultivation systems where the DO signal is used in measurement or control.