Antibacterial and anticancer characteristics of black carrot (Daucus Carota) extracts

摘要

The current research project is an attempt to investigate the antibacterial and anticancer traits of black carrot (Daucus Carota) extracts. The proximate composition, mineral profiling and antioxidant indices of D. Carota ethanol and acetone extracts were determined. Results revealed that the moisture content, total lipids, total ash, protein, and crude fiber contents of D. Carota (dry basis) were 16.4%, 0.94%, 12.4%, 1.14%, and 2.37%, respectively. Na, K and Ca contents were 65.32, 421.2 and 60.45 mg/100 g, respectively. High phenolic contents of black carrot were determined and accounted for 341.6 and 228.6 mg GAE/100 g FW for ethanol and acetone extracts, respectively. DPPH center dot and ferric reducing antioxidant power (FRAP) values of ethanol and acetone extracts were 31.7% & 2068 mg/100g, and 32.2% & 2027 mg/100g, respectively. Ethanol extract (1 g/mL) exhibited an inhibition zone of 6 and 8 mm against B. cereus and S. aureus, respectively. Black carrot extracts were screened for their anticancer activity against an MCF-7 (breast cancer) cell line by M7 assay. Using doxorubicin as positive control, results revealed that acetone and ethanol extracts (50 mu g/mL) displayed 8.13% and 30.6 % inhibition, respectively. These results showed that D. Carota extracts contain bioactive compounds that are effective against microbes and breast cancer proliferation.