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首页> 外文期刊>Helicobacter >GenoType? Helico DRDR test in comparison with histology and culture for Helicobacter pyloriHelicobacter pylori detection and identification of resistance mutations to clarithromycin and fluoroquinolones
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GenoType? Helico DRDR test in comparison with histology and culture for Helicobacter pyloriHelicobacter pylori detection and identification of resistance mutations to clarithromycin and fluoroquinolones

机译：基因型？ 与幽门螺杆菌的组织学和培养物相比，Helico Dr测试与幽门螺杆菌幽门螺杆菌幽门螺杆菌检测和抗性突变对克拉霉素和氟喹诺酮类的培养物相比

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Abstract <section numbered="no" xml:id="hel12447-sec-0001"> <title type="main">Background > There are several methods for Helicobacter pylori infection diagnosis. </section> <section numbered="no" xml:id="hel12447-sec-0002"> <title type="main">Aim > The efficacies of three methods for H. pylori identification directly from a biopsy were compared: histology, culture, and molecular GenoType? HelicoDR test. </section> <section numbered="no" xml:id="hel12447-sec-0003"> <title type="main">Materials & Methods >Eighty‐five triplicates of stomach antrum biopsies were obtained during gastroscopy procedures for culture, histology, and molecular assay. In addition, we performed molecular identification of genes encoding resistance to clarithromycin and fluoroquinolones. </section> <section numbered="no" xml:id="hel12447-sec-0004"> <title type="main">Results >The results have shown that the most specific method with the highest number of positive specimens was by molecular kit, compared to culture and histology (94.3%, 77.1%, and 71.4%, respectively). There was a higher rate of resistance mutations to clarithromycin than to fluoroquinolones (68.26% vs 20%). The most common mutations for clarithromycin and fluoroquinolones resistance were found in alleles A2143G and N87K, respectively. The highest rate of positive specimens was identified by the molecular. </section> <section numbered="no" xml:id="hel12447-sec-0005"> <title type="main">Discussion >GenoType HelicoDR kit (94.3%), which has several advantages: direct identification, strain resistance characterization </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：ID =“Hel12444-Abs-0001”XML：Lang =“en”> <标题类型=“main”>抽象</ title> <sectouned =“no”xml：id = “Hel12447-SEC-0001”> <标题类型=“main”>背景</标题> >有几种方法对于幽门螺杆菌</ i>感染诊断。 </ p> </ section> <部分编号=“否”XML：ID =“HEL12447-SEC-0002”> <标题类型=“main”> AIM </标题> >三种方法的效率<我> h。 Pylori </ i>直接从活组织检查的鉴定进行比较：组织学，培养和分子基因型？ Helicodr测试。 </ p> </ section> <secto numbyed =“no”xml：id =“hel12447-sec-0003”> <标题类型=“main”>材料＆amp;方法在胃镜检查过程中获得培养，组织学和分子测定的胃镜检查过程中获得胃窦活检的胃窦活组织检查八十五倍。此外，我们对编码含量的含量和氟喹诺酮类的基因进行了分子鉴定。</ p> </截面> <截面编号=“no”xml：id =“hel12447-sec-0004”> <标题类型=“main” >结果</标题> >结果表明，与培养物和组织学相比，分子试剂数量最多的阳性标本最多的方法（分别为94.3％，77.1％，分别为71.4％）。含氟霉素的抗性突变率较高，而不是氟喹啉（68.26％vs 20％）。 Clarithromycin和氟喹啉酮抗性的最常见突变分别在等位基因A2143G和N87K中发现。分子鉴定出阳性标本的最高速率。</ p> </ section> <secteneded =“no”xml：id =“hel12447-sec-0005”> <标题类型=“main”>讨论</标题> >基因型Helicodr套件（94.3％），具有几个优点：直接识别，应变电阻表征 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-32409/'>《Helicobacter》</a> <b style="margin: 0 2px;">|</b><span>2017年第6期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pastukh Nina&option=202" target="_blank" rel="nofollow">Pastukh Nina;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Binyamin Dana&option=202" target="_blank" rel="nofollow">Binyamin Dana;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=On Avi&option=202" target="_blank" rel="nofollow">On Avi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Paritsky Maya&option=202" target="_blank" rel="nofollow">Paritsky Maya;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peretz Avi&option=202" target="_blank" rel="nofollow">Peretz Avi;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Clinical Microbiology Laboratory Baruch Padeh Medical Center Poriya affiliated with the Faculty of MedicineGalilee Israel;</p> <p>Clinical Microbiology Laboratory Baruch Padeh Medical Center Poriya affiliated with the Faculty of MedicineGalilee Israel;</p> <p>The Faculty of Medicine in the GalileeBar Ilan UniversityZefat Israel;</p> <p>The Faculty of Medicine in the GalileeBar Ilan UniversityZefat Israel;</p> <p>Clinical Microbiology Laboratory Baruch Padeh Medical Center Poriya affiliated with the Faculty of MedicineGalilee Israel;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=clarithromycin&option=203" rel="nofollow">clarithromycin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=culture&option=203" rel="nofollow">culture;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fluoroquinolones&option=203" rel="nofollow">fluoroquinolones;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=GenoType? Helico DR test&option=203" rel="nofollow">GenoType? Helico DR test;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=histology&option=203" rel="nofollow">histology;</a> </p> <div class="translation"> 机译：克拉霉素;培养;氟喹诺酮类;基因型？ Helico Dr测试;组织学; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025099520.html">GenoType? 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C.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Calado Cecilia R. C. </a> <a href="/conference-foreign-196797/" target="_blank" rel="nofollow" class="tuijian_authcolor">1st Portuguese Meeting in Bioengineering .</a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过傅里叶变换红外光谱监测细菌过程：幽门螺杆菌药物失活和重组大肠杆菌培养物中的质粒生物生产</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061250454.html">Cholesterol-dependent and HefC-mediated mechanisms of bile salt resistance in Helicobacter pylori.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Trainor, Elizabeth Ashley.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Trainor, Elizabeth Ashley. </a> <span>2012</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: 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Kirabo,</a> <span>2006</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：来自乌干达消化性溃疡患者的幽门螺杆菌对克拉霉素和氟喹诺酮类药物高度耐药：GenoType HelicoDR测试结果直接应用于凳子</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100036573384.html ">Fast and Accurate Quantitative Detection of Helicobacter pylori and Identification of Clarithromycin Resistance Mutations in H. pylori Isolates from Gastric Biopsy Specimens by Real-Time PCR</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lascols, Christine&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lascols, Christine,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lamarque, Dominique&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lamarque, Dominique,</a> <a 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