Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis

摘要

A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the alpha1, alpha2, beta, gamma1, gamma2/theta, kappa, varepsilon, zeta, and iota intimin variants.