首页> 外文期刊>Veterinary Research Communications >Experimental study on allografts of amniotic epithelial cells in calcaneal tendon lesions of sheep.
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Experimental study on allografts of amniotic epithelial cells in calcaneal tendon lesions of sheep.

机译:绵羊肌腱病变中羊膜炎同种异体上皮细胞同种异体移植的实验研究。

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An experimental protocol was designed to study the survival and behaviour of an allograft of amniotic epithelial cells (AECs) in an ovine model. The study was conducted on three healthy adult sheep. A core lesion was created in both calcaneal tendons under ultrasound (US) guidance by injecting 400 UI of Type 1A collagenase diluted in 0.6 ml saline. The AECs were obtained from a 60-80-day-old fetus and cultured under standard conditions. After 15 days of collagenase treatment, 2x106 AECs stained with a vital membrane fluorescent probe (PHK26) were injected under US guidance in 500 micro l saline solution into the lesion of one limb. The contralateral untreated limb was used as a control. Animals were euthanatized 7 (1) and 30 (2) days later. Histological analyses performed on explanted tendons clearly demonstrate that AECs survived for at least 1 month inside the lesion without any adverse reactions. The damaged tissue of the treated tendons showed a high number of reparative cells in active proliferation that were accumulating collagen within the extracellular matrix. In addition, after 1 month, the neo-collagen began to be organized into parallel arrays of fibers oriented along the longitudinal axis of the tendon.
机译:设计实验方案,以研究绵羊模型中羊皮上皮细胞(AECS)的同种异体移植物的存活率和行为。该研究在三只健康成年羊上进行。通过在0.6ml盐水中稀释的1A型胶原酶的400 UI注入400 UI,在超声(US)指导下在两种钙肌腱中产生核心病变。 AECS是从60-80天的胎儿获得并在标准条件下培养。在15天后的胶原酶治疗后,用Vital膜荧光探针(PHK26)染色的2x10 6 在美国的指导下注射500微L盐水溶液的指导中的一个肢体。对侧未经处理的肢体用作对照。动物被安乐死7(1)和30(2)天后。在脱盐腱进行的组织学分析清楚地表明AECS在病变内部存活至少1个月,没有任何不良反应。经过处理的肌腱的受损组织显示出大量的可活性增殖的重复细胞,其在细胞外基质内积聚胶原。另外,在1个月之后,新胶原蛋白开始被组织成沿着肌腱的纵向轴线定向的平行纤维阵列。

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