Glucose Oxidation Catalyzed by Liposomal Glucose Oxidase in the Presence of Catalase-Containing Liposomes

摘要

A catalase-containing liposome (CAL)was prepared and characterized in terms of stability during storage and catalysis of the decomposition of hydrogen peroxide (H_2O_2)that was initially added or produced in the oxidation of glucose catalyzed by the glucose oxidase-containing liposomes (GOL).The reactors used were a test tube and an external loop airlift bubble column as the static liquid and circulating liquid flow systems,respectively.The free catalase (CA)at low concentrations was unstable during storage at 4 deg C as a result of dissociation of the tetrameric CA subunits.On the other hand,the deactivation of the CA activity in the CAL was depressed because of the high CA concentration in the CAL liposome.The CAL effectively catalyzed the repeated decompositions at 25 deg C with 10 mM H_2O_2 added initially,whereas the free CA was significantly deactivated during the repeated reactions.The high stability of the CAL was attributed to the moderately depressed reactivity,which was essentially derived from the diffusion limitation of the CAL membrane to H_2O_2 in the liquid bulk.In the GOL-catalyzed prolonged oxidation of 10 mM glucose at 40 deg C in the static liquid in a test tube,both the free CA and CAL could continuously catalyze the decomposition of H_2O_2 produced.This was because the glucose oxidation rate was small due to the limited reactivity of the GOL to glucose with its low permeability through the GOL membrane.In the glucose oxidation catalyzed by the GOL with the free CA or the CAL in the airlift,much larger oxidation rates were observed compared to those in the test tube because the permeability of the GOL membrane to glucose was increased in the gas-liquid two phase flow in the airlift.The GOL/CAL system in the airlift operated in an acidic condition,which was preferable to the GO activity,gave the largest oxidation rate with negligible accumulation of the H_2O_2 produced.On the other hand,the GOL/free CA system gave an oxidation rate smaller than that of the GOL/CAL system even under the acidic condition due to an unfavorable interaction of the free CA molecules with the GOL membranes leading to the decreased reactivity of the GOL.