Degradation of Pyrene Contaminated Soil with Spiked 14C Pyrene by Hemoglobin Catalysis

摘要

Hemoglobin (Hb) is a member of the hemeprotein family that undergoes non-specific catalytic chain reactions in the presence of hydrogen peroxide (H~(2)O~(2)). The catalytic ability of Hb to degrade the carcinogenic polycyclic aromatic hydrocarbon pyrene was demonstrated using soil contaminated with_(14)C pyrene. Three bench-scale laboratory tests were performed using_(14)C pyrene in the presence of a buffer, H~(2)O~(2), and a combination of Hb and H~(2)O~(2). The initial pyrene concentration of the contaminated soil was set to 11?mg/kg, with 5,500,000 dpm of_(14)C pyrene. After a catalytic reaction for 24?h, the results showed that 17% of pyrene was degraded by H~(2)O~(2), 38% of pyrene was degraded by Hb in combination with H~(2)O~(2), and 0.13 and 1.2% of_(14)C pyrene were mineralized by H~(2)O~(2)and Hb in combination with H~(2)O~(2), respectively. An analysis of the products from the reaction involving Hb in combination with H~(2)O~(2)revealed that 15.9% of the_(14)C intermediates in the acetonitrile fraction were polar products. After the catalytic reaction, 21 intermediate compounds were found via fraction analysis. The results suggested that Hb catalysis could be used to treat pyrene-contaminated soil as a novel catalytic technology for the remediation of hazardous materials in soil.