CMV seronegative donors: Effect on clinical severity of CMV infection and reconstitution of CMV-specific immunity

摘要

BackgroundCytomegalovirus (CMV)-specific T-cells are crucial to prevent CMV disease. CMV seropositive recipients transplanted with stem cells from a CMV seronegative allogeneic donor (R+D?) may be at risk for CMV disease due to absence of donor CMV-specific memory T-cells in the graft. MethodsWe analyzed the duration of CMV reactivations and the incidence of CMV disease in R+D?and R+D+patients after alemtuzumab-based T-cell depleted allogeneic stem cell transplantation (TCD alloSCT). To determine the presence of donor-derived primary CMV-specific T-cell responses we analyzed the origin of CMV-specific T-cells in R+D?patients. ResultsThe duration of CMV reactivations (54 versus 38?days, respectively, p?=?0.048) and the incidence of CMV disease (0.14 versus 0.02, p?=?0.003 at 1?year after alloSCT) were higher in R+D?patients compared to R+D+patients. In R+D?patients, CMV-specific CD4+and CD8+T-cells were mainly of recipient origin. However, in 53% of R+D?patients donor-derived CMV-specific T-cells were detected within the first year. ConclusionsIn R+D?patients, immunity against CMV was predominantly mediated by recipient T-cells. Nevertheless, donor CMV serostatus significantly influenced the clinical severity of CMV reactivations indicating the role of CMV-specific memory T-cells transferred with the graft, despite the ultimate formation of primary donor-derived CMV-specific T-cell responses in R+D?patients.