Effect of docosahexanoic acid on quality of frozen-thawed bull semen in BioXcell extender

摘要

This study was conducted to investigate the effect ofdocosahexanoic acid (DHA) supplementation in BioXcell extender on the quality of frozen-thawed bull semen. Twenty-four ejaculates were crtllccted from three bulls (eight from each bull). Ejaculates with motility ≥70% and normal morphology ≥80% were extende?) into BioXcell extender to which 0 (control), 3, 5, 10 or 15 ng mL~(-1) DHA was added. The supplemented semen samples were incubated at 37°C for 15 min fbr DHA uptake by spermatozoa. Later, samples were cooled for 2 h at 5°C and packaged into, 0.25-mL, straws, frozen in liquid nitrogen fbr 24 h and subsequently thawed tbr evaluatirm. Results are presented as percentages ± s.e.m. Supplementation with DllA at 3 ng mL~(-1) significantly improved spernt functional parameters including sperm motility, normal morphology, viability, acrosome integrity and membrane iittegrity when compared with other supplemented groups and the control. Lipid peroxidation increased as the incorporation of DHA supplementation increased. In conclusion, 3 ngmL~(-1) concentration of DHA resulted in superior quality of frozen-thawed bull spermatozoa and is suggested as the optimum level of DHA to be added into BioXcell extender.