首页> 外文期刊>Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics >Identification of a candidate gene underlying qKRN5b for kernel row number in Zea mays L.
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Identification of a candidate gene underlying qKRN5b for kernel row number in Zea mays L.

机译:在Zea 5月L中鉴定QKRN5B下面QKRN5B的候选基因。

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Key message A quantitative trait locus for kernel row number, qKRN5, was dissected into two tightly linked loci, qKRN5a and qKRN5b. Fine mapping, comparative analysis of nucleotide sequences and gene expression established the endonuclease/exonuclease/phosphatase family protein-encoding gene Zm00001d013603 as a causal gene of qKRN5b. Maize grain yield is determined by agronomically important traits that are controlled by interactions among and between genes and environmental factors. Considerable efforts have been made to identify major quantitative trait loci (QTLs) for yield-related traits; however, few were previously isolated and characterized in maize. In this study, we divided a QTL for kernel row number (KRN), qKRN5, into two tightly linked loci, qKRN5a and qKRN5b, using advanced backcross populations derived from near-isogenic lines. KRN was greater in individuals that were homozygous for the NX531 allele, which showed coupling-phase linkage. The major QTL qKRN5b had an additive effect of approximately one kernel row. Furthermore, fine mapping narrowed qKRN5b within a 147.2-kb region. The upstream sequence Zm00001d013603 and its expression in the ear inflorescence showed obvious differences between qKRN5b near-isogenic lines. In situ hybridization located Zm00001d013603 on the primordia of the spikelet pair meristems and spikelet meristems, but not in the inflorescence meristem, which indicates a role in regulating the initiation of reproductive axillary meristems of ear inflorescences. Expression analysis and nucleotide sequence alignment revealed that Zm00001d013603, which encodes an endonuclease/exonuclease/phosphatase family protein that hydrolyzes phosphatidyl inositol diphosphates, is the causal gene of qKRN5b. These results provide insight into the genetic basis of KRN and have potential value for enhancing maize grain yield.
机译:关键消息用于内核数量的数量特质QKRN5,QKRN5被解剖到两个紧密连接的基因座,QKRN5A和QKRN5B中。细微映射,对核苷酸序列和基因表达的比较分析使内切核酸酶/外核酸酶/磷酸酶家族蛋白质编码基因ZM00001D013603作为QKRN5B的因果基因。玉米籽粒产量由农艺上的重要特征决定,该特征是基因和环境因素之间的相互作用控制。已经进行了相当大的努力来确定有关产量相关性状的主要定量特质基因座(QTL);然而,少数以前孤立和特征在玉米中。在这项研究中,我们将QTL划分为内核行号(KRN),QKRN5,进入两个紧密连接的基因座,QKRN5A和QKRN5B,使用源自近代源线的先进的回复群体。 Krn在均匀的NX531等位基因纯合的个体中更大,显示偶联相连。主要QTL QKRN5B具有大约一个内核行的添加效果。此外,在147.2-kB区域内精细映射缩小了QKRN5B。上游序列ZM00001D013603及其在耳开口中的表达在QKRN5B近代线之间显示出明显的差异。原位杂交位于ZM00001D013603对穗子弹分型分词和小穗分泌的原序,但不是在花序分生物中,这表明在调节耳廓开采的生殖腋窝分析开始方面的作用。表达分析和核苷酸序列对准显示,ZM00001D013603编码了水解磷脂酰二磷酸二磷酸溶磷脂酰基磷酸酯的内切核酸酶/外切核酸酶/磷酸酶家族蛋白质,是QKRN5B的因果基因。这些结果提供了对KRN的遗传基础的洞察力,并且具有增强玉米籽粒产量的潜在价值。

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    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430030 Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430030 Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430030 Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430030 Hubei Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430030 Hubei Peoples R China;

    Yangtze Univ Hubei Collaborat Innovat Ctr Grain Crops Jingzhou 434025 Peoples R China;

    Huazhong Agr Univ Natl Key Lab Crop Genet Improvement Wuhan 430030 Hubei Peoples R China;

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  • 正文语种 eng
  • 中图分类 医学遗传学;
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  • 入库时间 2022-08-20 07:32:06

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