首页> 外文期刊>Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics >A major QTL and candidate genes for capsaicinoid biosynthesis in the pericarp of Capsicum chinense revealed using QTL-seq and RNA-seq
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A major QTL and candidate genes for capsaicinoid biosynthesis in the pericarp of Capsicum chinense revealed using QTL-seq and RNA-seq

机译:在辣椒和RNA-SEQ揭示Capsicum Chinense的Pericarp中的主要QTL和候选基因生物合成,揭示了QTL-SEQ和RNA-SEQ

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Key messageA major QTL and candidate genes controlling capsaicinoid content in the pericarp were identified by QTL-seq and RNA-seq in Capsicum chinense.AbstractCapsaicinoid biosynthesis was previously thought to be restricted to the placental tissue; however, the recent discovery of their biosynthesis in the pericarp provides new opportunities to increase the capsaicinoid content in pepper fruits. Currently, the genetic mechanisms regulating capsaicinoid biosynthesis in the pericarp remain unknown. Here, we performed quantitative trait loci (QTL) mapping and RNA sequencing (RNA-seq) to reveal the genes controlling capsaicinoid biosynthesis in the pericarp. A whole-genome sequencing-based QTL-seq strategy was employed, identifying a major QTL on chromosome 6. To validate the QTL on chromosome 6, we performed traditional QTL mapping using the same population in QTL-seq with an additional biparental population. A total of 15 QTLs for capsaicinoid content distributed on chromosomes 3, 6, and 11 were newly identified. Among these QTLs, the genetic loci on the lower arm of chromosome 6 were commonly detected in the two mapping populations, corresponding to the location of the major QTL detected using whole-genome sequencing-based QTL-seq. Our RNA-seq analysis identified candidate genes within the common QTL that were differentially expressed in the pungent and non-pungent pericarp tissues. Our results are expected to contribute to the elucidation of the regulation of capsaicinoid biosynthesis. We also demonstrated that a combination of QTL mapping and RNA-seq is helpful for refining the candidate genes of a complicated trait of interest.
机译:QTL-SEQ和控制果皮中的辣椒素含量的主要QTL和候选基因被QTL-SEQ和RNA-SEQ鉴定在Capsicum Chinense.AbstractCAPSIcinoinaInoid生物合成中,先前被认为是限于胎盘组织;然而,最近在Pericarp中发现它们的生物合成提供了新的机会,可以增加胡椒水果中的辣椒素含量。目前,遗传机制调节Pericarp中的胶囊化生物合成仍然未知。这里,我们进行了定量性状基因座(QTL)映射和RNA测序(RNA-SEQ),以揭示控制Pericarp中的控制辣椒素生物合成的基因。采用全基因组测序的基于QTL-SEQ策略,鉴定染色体6上的主要QTL。为了验证染色体6上的QTL,我们使用QTL-SEQ中的相同群体进行了传统的QTL映射,并具有额外的千分之一群。新鉴定了分布在染色体3,6和11上的15个QTL用于分布在染色体3,6和11上的胶囊含量。在这些QTL中,染色体6的下臂上的遗传基因座通常在两个映射群体中检测到,对应于使用基于全基因组测序的QTL-SEQ检测的主要QTL的位置。我们的RNA-SEQ分析确定了常见QTL内的候选基因,该基因在刺鼻和非刺激性卵皮肤组织中差异表达。我们的结果预计将有助于阐明胶囊化生物合成的调节。我们还证明了QTL映射和RNA-SEQ的组合有助于精制感兴趣的复杂性状的候选基因。

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