首页> 外文期刊>Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics >Identifying a wild allele conferring small seed size, high protein content and low oil content using chromosome segment substitution lines in soybean
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Identifying a wild allele conferring small seed size, high protein content and low oil content using chromosome segment substitution lines in soybean

机译:使用大豆中的染色体分部替代线识别赋予小种子大小,高蛋白质含量和低油含量的野生等位基因

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Annual wild soybean characterized with small 100-seed weight (100SW), high protein content (PRC), low oil content (OIC) may contain favourable alleles for broadening the genetic base of cultivated soybeans. To evaluate these alleles, a population composed of 195 chromosome segment substitution lines (SojaCSSLP4), with wild N24852 as donor and cultivated NN1138-2 as recurrent parent, was tested. In SojaCSSLP4, 10, 9 and 8 wild segments/QTL were detected for 100SW, PRC and OIC, respectively. Using a backcross-derived secondary population, one segment for the three traits (q100SW15, qPro15 and qOil15) and one for 100SW (q100SW18.2) were fine-mapped into a 329-kb region on chromosome 15 and a 286-kb region on chromosome 18, respectively. Integrated with the transcription data in SoyBase, 42 genes were predicted in the 329-kb region where Glyma.15g049200 showed significant expression differences at all seed development stages. Furthermore, the Glyma.15g049200 segments of the two parents were sequenced and compared, which showed two base insertions in CDS (coding sequence) in the wild N24852 comparing to the NN1138-2. Since only Glyma.15g049200 performed differential CDS between the two parents but related to the three traits, Glyma.15g049200 was predicted a pleiotropic candidate gene for 100SW, PRC and OIC. The functional annotation of Glyma.15g049200 indicated a bidirectional sucrose transporter belonging to MtN3/saliva family which might be the reason that this gene provides a same biochemical basis for 100SW, PRC and OIC, therefore, is responsible for the three traits. This result may facilitate isolation of the specific gene and provide prerequisite for understanding the other two pleiotropic QTL.
机译:每年野生大豆具有小的100种重量(100sW),高蛋白质含量(PRC),低油含量(OIC)可含有有利的等位基因,用于拓宽栽培大豆的遗传基础。为了评估这些等位基因,测试了由195种染色体分段取代线(SOJACSSLP4)组成的群体,作为供体的野生N24852和培养NN1138-2作为复发父母。在SOJACSSLP4,10,9和8个野生区段/ QTL分别被检测到100SW,PRC和OIC。使用回溯衍生的二次群体,三个特征(Q100SW15,QPro15和Qoil15)的一个段和100SW(Q100SW18.2)被精细映射到染色体15和286-KB区域上的329-Kb区域染色体18分别。与大豆中的转录数据集成,在329-KB区域预测了42个基因,其中Glyma15G049200在所有种子发育阶段显示出显着的表达差异。此外,对两个父母的细胞进行测序并进行比较,其在野生N24852中显示CDS(编码序列)中的两个碱基插入,与NN1138-2相比。由于只有Glyma.15G049200在两个父母之间进行差异CD,但与三个性状相关,因此Glyma15G049200预测了100SW,PRC和OIC的抗磷酸候选基因。 Glyma.15G049200的功能注释表明了属于MTN3 /唾液的双向蔗糖转运蛋白,这可能是该基因为100SW,PRC和OIC提供相同的生化基础,因此负责三个特征。该结果可以促进特定基因的分离,并提供理解其他两种磷酸QTL的先决条件。

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