首页> 外文期刊>Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics >Characterization of black spot resistance in diploid roses with QTL detection, meta-analysis and candidate-gene identification
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Characterization of black spot resistance in diploid roses with QTL detection, meta-analysis and candidate-gene identification

机译:QTL检测,荟萃分析和候选基因鉴定二倍体玫瑰黑点抗性的表征

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Key message Two environmentally stable QTLs linked to black spot disease resistance in theRosa wichuranagenetic background were detected, in different connected populations, on linkage groups 3 and 5. Co-localization betweenR-genes and defense response genes was revealed via meta-analysis. The widespread rose black spot disease (BSD) caused by the hemibiotrophic fungusDiplocarpon rosaeWolf. is efficiently controlled with fungicides. However, in the actual context of reducing agrochemical use, the demand for rose bushes with higher levels of resistance has increased. Qualitative resistance conferred by major genes (Rdrgenes) has been widely studied but quantitative resistance to BSD requires further investigation. In this study, segregating populations connected through the BSD resistantRosa wichuranamale parent were phenotyped for disease resistance over several years and locations. A pseudo-testcross approach was used, resulting in six parental maps across three populations. A total of 45 individual QTLs with significant effect on BSD resistance were mapped on the male maps (on linkage groups (LG) B3, B4, B5 and B6), and 12 on the female maps (on LG A1, A2, A3, A4 and A5). Two major regions linked to BSD resistance were identified on LG B3 and B5 of the male maps and were integrated into a consensus map built from all three of the male maps. A meta-analysis was used to narrow down the confidence intervals of individual QTLs from three populations by generating meta-QTLs. Two 'hot spots' or meta-QTLs were found per LG, enabling reduction of the confidence interval to 10.42 cM for B3 and 11.47 cM for B5. An expert annotation of NBS-LRR encoding genes of the genome assembly of Hibrand et al. was performed and used to explore potential co-localization withR-genes. Co-localization with defense response genes was also investigated.
机译:关键消息在不同连接的群体中检测到与黑点疾病抗病患者有关患有黑点疾病抗性的环境稳定QTL,在不同的连接群体中,通过META分析揭示了与基因之间的共定位和防御反应基因。由血管性营养菌引起的广泛玫瑰黑点疾病(BSD)引起的血红蛋白罗斯罗斯罗斯电子罗斯。用杀菌剂有效地控制。然而,在减少农业化学利用的实际背景下,耐抗含量较高的玫瑰灌木的需求增加。主要基因(RDRENES)赋予的定性抗性已被广泛研究,但对BSD的定量抵抗需要进一步调查。在这项研究中,通过BSD抗抵抗替氏菌的分离群体在几年和地点患上抗病性的表型。使用了伪测试方法,导致三个群体中的六个父母地图。共有45个对BSD抗性作用显着影响的单独QTL映射在雄性贴图上(在连接基团(LG)B3,B4,B5和B6)和女性地图上(在LG A1,A2,A3,A4上进行12个和a5)。在男性地图的LG B3和B5上鉴定了与BSD抗性相关的两个主要区域,并融入了由三个男性地图构建的共识图。通过产生Meta-QTLS来利用META分析来缩小三个群体的单个QTL的置信区间。每个LG发现两个'热点'或Meta-QTL,使B3和11.47cm的B3和11.47cm的置信区间降低至10.42cm。 Hibrand等人基因组组装NBS-LRR编码基因的专家注释。进行并用于探索潜在的共同定位与基因。还研究了与防御反应基因的共同定位。

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