Cumulus-oocyte interactions and programmed cell death in bovine embryos produced in vitro

摘要

This study investigated the interactions between cumulus cells (CCs) and oocytes and programmed cell death in bovine cumulus-oocyte complexes (COCs) with different morphological characteristics. DNA fragmentation was assessed in CCs at 0 and 24 h of maturation, as well as parthenogenetic developmental competence on the 9th day post-activation, blastocyst quality and BCL-2 and BAX transcript levels in matured CCs. Most immature oocytes in the COC-A group (full cumulus and several compact layers) were in the initial germinal vesicle (iGV) stage, exhibiting minimal or no DNA damage. In contrast, after follicle removal, the COC-B (partial cumulus and one or two cell layers) and -C (expanded cumulus) groups presented in more advanced GV stages and exhibited DNA fragmentation. After maturation, significant increases in fragmented nuclei were noted in COC-C and COC-B groups. Embryos resulting from the COC-A developed more rapidly and had increased competence compared to embryos resulting from groups COC-B and COC-C. The COC-B group exhibited the highest BAX protein levels and a reduced BCL-2/BAX protein ratio. The results show a negative correlation between nuclear fragmentation and embryonic development potential in COCs with different morphologies. In addition, a low BCL-2/ BAX protein ratio might be associated with an increase in nuclear fragmentation in CCs. (C) 2018 Elsevier Inc. All rights reserved.