DICROCOELIUM DENDRITICUM INFECTION AMONG LIVESTOCK IN WESTERN IRAN

摘要

Dicrocoelium, also known as liver fluke, is responsible for dicrocoeliasis in humans and many herbivores. Given the importance of medical, veterinary, health, and economics of dicrocoeliasis, the current study sought to identify D. dendriticum by PCR amplification of NADH dehydrogenase subunit gene (nad1) (900 bp) followed by Rsa-1digestion (206 and 694 bp) from liver of sheep, cows and goats (15 per group) in Ilam, Lorestan and Kermanshah Provinces, Iran. Of 45 D. dendriticum isolates identified (one per animal), 7 amplicons (900 bp) were verified by direct DNA sequencing.