Evaluation of Emdogain (R) antimicrobial effectiveness against biofilms containing the keystone pathogen Porphyromonas gingivalis

摘要

This study aimed to evaluate the antimicrobial activity of Emdogain (R) (EMD) against biofilms containing the periopathogen Porphyromonas gingivalis. A brain-Heart infusion broth inoculated with S. gordonii and P. gingivalis was perfused (7-d, anaerobiosis) through a closed circuit containing two Robbins devices as to form biofilms. The latter were then treated for 2 min with various antimicrobials (Chlorhexidine (CHX) 0.2%, Povidone iodine (PVI) 5%, PVI 10%, essential oils (EO), EO Zero (TM) or EMD) (n=8) and cell densities were calculated and compared. In the present in vitro model, Emdogain (R) was not statistically effective (p0.05) in killing biofilm bacteria unlike the other tested molecules.