Sex-linked AFLP marker identification in dioecious Betelvine (Piper betle L.)

摘要

This study aimed to develop sex-specific AFLP markers for dioecious Piper betle (Betelvine). A combination of ulk segregant analysis (BSA) and non-radioactive labelling led to simple, rapid and reliable PCR-based screening of sex-specific markers. A total of nine primer combinations were screened using BSA for male and female cultivars. Seven out of nine primer combinations produced bands specific to either of the sex. These seven primer combinations when used for screening DNA sample from individual male and female cultivars yielded two primer combinations which produced male sex-specific markers. Primer combinations EcoRI-CTA/MseI-CTA and EcoRI-CGT/MseI-CGT produced unique fragments of similar to 147 bp and similar to 326 bp, respectively, in bulk DNA as well as in DNA sample from individual male and female cultivars. These unique fragments were present in all the tested males and absent from all the tested female cultivars.