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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Effects of abscisic acid agonist or antagonist applications on aroma volatiles and anthocyanin biosynthesis in grape berries
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Effects of abscisic acid agonist or antagonist applications on aroma volatiles and anthocyanin biosynthesis in grape berries

机译:脱离酸激动剂或拮抗剂应用对葡萄浆果芳香挥发物和花青素生物合成的影响

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摘要

The effects of applying abscisic acid (ABA), Abz (abscinazole, an inhibitor of CYP707A), and nordihydroguaiaretic acid ((NDGA), an inhibitor of 9-cis-epoxycarotenoid dioxygenase (NCED)) on fruit firmness, anthocyanin biosynthesis, aroma volatiles, and their related genes were investigated in grape berries (Vitis vinifera L. x Vitis labrusca L. cv. Kyoho). ABA and Abz application promoted the accumulation of anthocyanin and aroma volatiles, the activity of polygalacturonase (PG), pectin methylesterase, and cellulase, but reduced fruit firmness. On the other hand, NDGA showed the opposite tendencies. Abz decreased VvCYP707A1 expression but increased VvPYL8 expression. VvPP2C49 expression was negatively correlated with ABA concentration. The expression levels of VvMYBA2 and VvUFGT correlated with anthocyanin concentration, unlike those of VvLAR1. ABA and Abz application increased endogenous ABA concentrations and the expression levels of VvOPR3, VvACO1, and VvPG, whereas NDGA application decreased them. These results suggest that ABA concentrations influence anthocyanin formation and fruit softening through the expression of VvMYBA2, VvUFGT, and VvPG.
机译:应用脱盐酸(ABA),ABZ(CYP707A)和Nordihoguaiaretic acid酸((NDGA),9-顺式环氧丙酮蛋白二氧化根蛋白(NCED)抑制剂的疗效效果对果实固态,花青素生物合成,香气挥发物并且在葡萄浆果中研究了它们的相关基因(血管伏苦氏菌毒性L. vitis Labrusca L.CV。kyoho)。 ABA和ABZ应用促进了花青素和香气挥发物的积累,聚乳糖酰胺酶(PG),果胶甲基酯酶和纤维素酶的活性,但减少了果实的坚定性。另一方面,NDGA表现出相反的趋势。 ABZ减少了VVCYP707A1表达,但增加了VVPYL8表达。 VVPP2C49表达与ABA浓度呈负相关。与vvlar1的那些,与花青素浓度相关的VVMYBA2和VVUFGT的表达水平。 ABA和ABZ应用增加了内源性ABA浓度和VVVOPR3,VVACO1和VVPG的表达水平,而NDGA应用降低了它们。这些结果表明,ABA浓度通过VVMYBA2,VVUFGT和VVPG的表达影响花青素形成和果实软化。

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