Molecular mapping of nuclear male-sterility gene ms-1 in muskmelon (Cucumis melo L.)

摘要

Five recessive and non-allelic genes, ms-1, ms-2, ms-3, ms-4 and ms-5 possessing unique phenotypes govern male sterility in muskmelon. An inbred MS-1, having recessive ms-1 gene, refined at Punjab Agricultural University, Ludhiana (India) has been successfully utilised for the development of commercial F-1 hybrids. Since the transfer of a recessive gene is tedious and time-consuming, it is very difficult to incorporate the male-terility gene, ms-1 in different backgrounds. The current study was conducted to construct a simple sequence repeat (SSR) marker-based linkage map for ms-1 locus using F-2 population derived from a cross 'MS-1 // KP4HM-15MODIFIER LETTER PRIME. A total of 506 SSR primer pairs was screened followed by bulked segregant analysis (BSA). Linkage analysis of 150 F-2 plants mapped three SSR markers, DM0187, DM0038 and TJ14 linked to the ms-1 gene on chromosome 6. The marker, DM0187 was closest to the gene at a genetic distance of 4.8 cM. The identified marker can be effectively used to transfer ms-1 gene into elite muskmelon genotypes through marker-assisted backcross breeding, till more tightly linked markers are available. Further, determination of its chromosomal position will be useful for the fine mapping and also provide a basis for cloning of the gene, ms-1.