Novel high-throughput screening method using quantitative PCR to determine the antimicrobial susceptibility of Orientia tsutsugamushi clinical isolates

Phuklia Weerawat; Panyanivong Phonepasith; Sengdetka Davanh; Sonthayanon Piengchan; Newton Paul N.; Paris Daniel H.; Day Nicholas P. J.; Dittrich Sabine

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Novel high-throughput screening method using quantitative PCR to determine the antimicrobial susceptibility of Orientia tsutsugamushi clinical isolates

机译：使用定量PCR的新型高通量筛选方法确定东方Tsutsugamushi临床分离株的抗微生物易感性

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Objectives: To develop a method to enable the large-scale antimicrobial susceptibility screening of Orientia tsutsugamushi clinical isolates, using one timepoint and one concentration of antibiotics to considerably speed up the time to result.

机译：目的：开发一种方法，以实现Orientia Tsutsugamushi临床分离物的大规模抗微生物易感性筛查，使用一种时间点和一种浓度的抗生素来显着加速结果。

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来源
《The Journal of Antimicrobial Chemotherapy》 |2019年第1期|共8页
作者
Phuklia Weerawat; Panyanivong Phonepasith; Sengdetka Davanh; Sonthayanon Piengchan; Newton Paul N.; Paris Daniel H.; Day Nicholas P. J.; Dittrich Sabine;
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作者单位

Mahidol Univ Fac Trop Med Dept Mol Trop Med &

Genet Bangkok Thailand;

Mahosot Hosp Microbiol Lab Lao Oxford Mahosot Hosp Wellcome Trust Res Unit L Viangchan Laos;

Mahosot Hosp Microbiol Lab Lao Oxford Mahosot Hosp Wellcome Trust Res Unit L Viangchan Laos;

Mahidol Univ Fac Trop Med Dept Mol Trop Med &

Genet Bangkok Thailand;

Mahosot Hosp Microbiol Lab Lao Oxford Mahosot Hosp Wellcome Trust Res Unit L Viangchan Laos;

Mahidol Univ Fac Trop Med Mahidol Oxford Trop Med Res Unit Bangkok Thailand;

Mahidol Univ Fac Trop Med Mahidol Oxford Trop Med Res Unit Bangkok Thailand;

Mahosot Hosp Microbiol Lab Lao Oxford Mahosot Hosp Wellcome Trust Res Unit L Viangchan Laos;

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原文格式 PDF
正文语种 eng
中图分类治疗学;
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专利

1. Novel high-throughput screening method using quantitative PCR to determine the antimicrobial susceptibility of Orientia tsutsugamushi clinical isolates [J] . Phuklia Weerawat, Panyanivong Phonepasith, Sengdetka Davanh, The Journal of Antimicrobial Chemotherapy . 2019,第1期

机译：使用定量PCR的新型高通量筛选方法确定东方Tsutsugamushi临床分离株的抗微生物易感性
2. Application of Monoclonal Antibody, Specific for Intracellular Orientia tsutsugamushi, to Immunofluorescent Antibody Test for Determining Antibiotic Susceptibility. [J] . Kim MK, Odgerel Z, Kim MJ, Microbiology and Immunology . 2004,第9期

机译：专用于细胞内Or虫东方定向的单克隆抗体在免疫荧光抗体检测中确定抗生素敏感性的应用。
3. A highly sensitive quantitative real-time PCR assay based on the groEL gene of contemporary Thai strains of Orientia tsutsugamushi. [J] . Paris DH, Aukkanit N, Jenjaroen Clinical microbiology and infection: European Society of Clinical Microbiology and Infectious Diseases . 2009,第5期

机译：基于当代泰国Or虫病东方株的groEL基因的高灵敏定量实时PCR分析。
4. Comparative Proteomic Analysis ofAntibiotic-Sensitive and Insensitive Isolates of Orientia tsutsugamushi [C] . Chien-Chung Chao, Donita L. Garland, Gregory A. Dasch, International Conference on Rickettsiae and Rickettsial Diseases . 2009

机译：堇青树敏感和不敏感分离株的对比蛋白质组学分析
5. Staphylococcus leei: Metabolic profile; antimicrobial susceptibility; purification of microbial urease; cloning, sequencing and expression of the urease gene in Escherichia coli; development of ELISA and PCR methods to determine the incidence of infection. [D] . Jin, Ming. 2002

机译：李氏葡萄球菌：代谢概况；抗菌药敏感性纯化微生物脲酶；脲酶基因的克隆，测序及在大肠杆菌中的表达ELISA和PCR方法的发展，以确定感染的发生率。
6. Novel high-throughput screening method using quantitative PCR to determine the antimicrobial susceptibility of Orientia tsutsugamushi clinical isolates [O] . Weerawat Phuklia, Phonepasith Panyanivong, Davanh Sengdetka, -1

机译：定量PCR的新型高通量筛选方法用于测定虫病东方菌临床分离株的抗菌药敏性
7. Comparison of Four Antimicrobial Susceptibility Testing Methods To Determine the In Vitro Activities of Piperacillin and Piperacillin-Tazobactam against Clinical Isolates of Enterobacteriaceae and Pseudomonas aeruginosa [O] . Karlowsky, James A., Weaver, Mellany K., Thornsberry, Clyde, 2003

机译：确定哌拉西林和哌拉西林-他唑巴坦对肠杆菌科和绿脓杆菌临床分离株体外活性的四种抗菌药敏试验方法的比较
8. Short Report: Detection of Orientia Tsutsugamushi in Clinical Samples by Quantitative Real-Time Polymerase Chain Reaction [R] . Singhsilarak, T. , Leowattana, W. , Looareesuwan, S. , 2005

机译：简短报道：通过定量实时聚合酶链反应检测临床样品中的恙虫病东方体

Novel high-throughput screening method using quantitative PCR to determine the antimicrobial susceptibility of Orientia tsutsugamushi clinical isolates

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