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首页> 外文期刊>Advances in virology >Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan
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Genotyping of HCV RNA Reveals That 3a Is the Most Prevalent Genotype in Mardan, Pakistan

机译:HCV RNA的基因分型显示巴基斯坦的马尔丹3a是最普遍的基因型

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摘要

The clinical outcomes of patients infected with hepatitis C virus (HCV) range from acute resolving hepatitis to chronic liver diseases such as liver cirrhosis or hepatocellular carcinoma. Identification of the infecting virus genotype is indispensable for the exploration of many aspects of HCV infection, including epidemiology, pathogenesis, and response to antiviral therapy. 1419 individuals were screened for anti-HCV in this study, of which 166 (11.7%) were found reactive by ICT (Immunochromatographic test). These 166 anti-HCV positive and 26 normal individuals were further analyzed. RNA was extracted from serum and reverse-transcribed to cDNA and the core region of HCV genome was targeted and amplified by multiplex PCR. HCV RNA was detected in 121 individuals, of which 87 were male and 34 were female. Genotype 3a was the most prevalent among all the genotypes observed followed by 3b. Genotypes la, 2a, and 2b were found in 10.89%, 13.22%, and 6.61% patients, respectively. 25.41% of the HCV RNA positive samples were not typed. 6.05% of patients were found having mixed genotypes. These findings will not only help the physicians to prescribe more appropriate treatment for the HCV infection but will also draw the attention of health-related policy makers to devise strategies to curb the disease more effectively.
机译:感染丙型肝炎病毒(HCV)的患者的临床结局范围从急性消退性肝炎到慢性肝病,例如肝硬化或肝细胞癌。感染性病毒基因型的鉴定对于探索HCV感染的许多方面都是必不可少的,包括流行病学,发病机理和对抗病毒治疗的反应。在这项研究中筛选了1419例抗HCV个体,其中166例(11.7%)通过ICT(免疫色谱测试)发现有反应。这166名抗HCV阳性和26名正常个体被进一步分析。从血清中提取RNA并逆转录为cDNA,并通过多重PCR靶向并扩增HCV基因组的核心区域。在121个人中检测到HCV RNA,其中男性87人,女性34人。在观察到的所有基因型中,基因型3a最普遍,其次是3b。基因型1a,2a和2b分别见于10.89%,13.22%和6.61%的患者。 25.41%的HCV RNA阳性样品未分型。发现6.05%的患者具有混合基因型。这些发现不仅将帮助医生为HCV感染开出更合适的治疗方法,还将引起与健康有关的决策者的注意,以制定更有效地遏制该疾病的策略。

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