Advanced Accuracy and Precision in Nanolitre Microinjection

摘要

Single-cell microinjection is a powerful and versatile technique for introducing exogenous material into cells, or transferring cellular components between cells. It is normally performed using a fine glass capillary, under a specialised optical microscope setup incorporating a micromanipulator. Microinjection is frequently used in genetic engineering and transgenetics to insert genetic material into single cells. It is also used in basic genetics research, in the cloning of organisms, and in the study of cell biology and viruses. Common clinical applications include In Vitro Fertilisation (IVF) and Intracytoplasmic Sperm Injection (ICSI) to help achieve fertilisation for couples with severe male factor infertility. Other areas include ophthalmology procedures such as Retinal Pigment Epithelial (RPE) and Intra Occular (IO) injection.