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Cardiac procholecystokinin expression during haemodynamic changes in the mammalian heart

机译:哺乳动物心脏血液动力学变化期间的心脏procholecystokinin表达

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摘要

Cardiac myocytes express the cholecystokinin gene (CCK) at propeptide level. We recently reported that cardiac CCK expression is acutely regulated by isoprenaline in a porcine model.The regulation of CCK expression after myocardial infarction, in exercise, and in severe heart failure is, however, unknown. Cardiac tissue was obtained from healthy new-born and adolescent farm pigs. Myocardial infarction was induced by coronary artery occlusion in adult minipigs. Healthy male subjects performed a 3-hour exercise test, and patients with severe heart failure referred for right heart catheterization were included. Extracts of porcine cardiac tissue and human plasma were analysed with specific proCCK radioimmunoassays. Cardiac proCCK expression shifted from the right atrium in new-born piglets to include the left atrium in adolescent pigs. Regional proCCK expression in the adolescent pig heart was mainly confined to the atria without different expression in sinus node tissue. In adult minipigs with myocardial infarction, no changes in overall left ventricular function or proCCK expression were observed after 8 weeks. In healthy adults, proCCK in circulation increased markedly during exercise in parallel with pro-B-type natriuretic peptide. Finally, patients with severe heart failure displayed markedly increased proCCK – but not CCK – concentrations in plasma. Taken together, our data shows that regional proCCK expression reflects haemodynamic changes in the mammalian heart. The data supports the notion that cardiac CCK expression resembles that of cardiac natriuretic peptides in atria. The ventricular content of proCCK, however, differs from natriuretic peptides and suggests a distinct secretory pathway in ventricular cardiomyocytes.
机译:心肌细胞在铅化水平下表达胆囊蛋白基因(CCK)。我们最近报道,心脏CCK表达是猪模型中异丙醇的敏感调节。然而,心肌梗死后CCK表达的调节,运动和严重的心力衰竭是未知的。心脏组织是从健康的新生和青少年农场猪中获得的。成人微型剂中的冠状动脉闭塞诱导心肌梗死。健康的男性受试者进行了3小时的运动试验,包括右心导管的严重心力衰竭的患者。用特定的ProCCK放射免疫测定分析猪心脏组织和人血浆的提取物。心脏procck表达从新出生的仔猪中的右心中移位,以包括青少年猪的左心房。青少年猪心脏的区域Procck表达主要被鼻窦组织中没有不同表达的阿里亚。在具有心肌梗死的成人微型剂中,8周后没有观察到总体左心室功能或ProCck表达的变化。在健康的成年人中,与Pro-B型利钠肽平行的运动期间,循环的Procck在运动中显着增加。最后,严重心力衰竭患者显示出显着增加的ProCck - 但不是血浆中的CCK浓度。我们的数据显示,区域Procck表达反映了哺乳动物心脏的血液动力学变化。该数据支持心脏CCK表达类似于Atria的心脏Natrietic肽的概念。然而,Procck的心室含量不同于利钠肽,并表明室心心肌细胞中的不同分泌途径。

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  • 来源
    《Peptides: An International Journal》 |2018年第2018期|共7页
  • 作者单位

    Department of Clinical Biochemistry Copenhagen University Hospital Rigshospitalet;

    Department of Clinical Biochemistry Copenhagen University Hospital Rigshospitalet;

    Department of Clinical Biochemistry Copenhagen University Hospital Rigshospitalet;

    Department of Clinical Biochemistry Copenhagen University Hospital Rigshospitalet;

    Department of Cardiology Bispebjerg Hospital;

    Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of;

    Department of Technology Metropolitan University College;

    Department of Clinical Biochemistry Copenhagen University Hospital Rigshospitalet;

    Department of Clinical Biochemistry Copenhagen University Hospital Rigshospitalet;

    Department of Clinical Biochemistry Copenhagen University Hospital Rigshospitalet;

    Department of Cardiology Copenhagen University Hospital Rigshospitalet;

    Department of Cardiology Copenhagen University Hospital Rigshospitalet;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    ANP; BNP; CCK; Cholecystokinin; Heart failure; Natriuretic peptide;

    机译:anp;bnp;cck;cck;cholecystokinin;心力衰竭;利钠肽;

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