Exogenous application of melatonin mitigates the adverse effects of drought stress on morpho-physiological traits and secondary metabolites in Moldavian balm (Dracocephalum moldavica)

摘要

Melatonin has emerged as an important signaling molecule that regulates plant responses to environmental stresses. In this research, melatonin was used to alleviate the adverse effects of oxidative stress induced by water deficit in Moldavian balm (Dracocephalum mol-davica) plants and morpho-physiological traits were investigated. This experiment was conducted as a factorial arrangement based on completely randomized design with four replications. Treatments included foliar melatonin application at four levels 0 (distilled water), 50, 100 and 150 uM and drought stress 100 (control), 80, 60 and 40% of field capacity (FC). Higher levels of drought stress at 60% and 40% FC, caused the reduction of plant height, shoot fresh and dry weight, root length,root fresh and dry weight, photosynthetic pigments and protein content. Increased amount of soluble sugar content, malondialde-hyde content and lipoxygenase activity, non-enzyme antioxidants (including flavonoid, polyphenol compounds and anthocyanin), phenylalanine ammonia-lyase and polyphenol oxidase enzymes activities were also observed at 60% and 40% FC. Melatonin at 100 uM improved I morphological parameters, photosynthetic pigments and protein content under moderate and severe drought stress. The obtained results suggested that foliar application of 100 muM melatonin also alleviated oxidative burst and malondialdehyde production in Moldavian balm plant under moderate and severe drought stress probably through regulation of secondary metabolism andthe enzymes activity of phenylalanine ammonia-lyase and polyphenol oxidase.