A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells

摘要

Cladosporium herbarum is a plant pathogen associated with passion fruit scab and mild diseases in pea and soybean. In this study, a peptidogalactomannan (pGM) of C. herbarum mycelium was isolated and structurally characterized, and its role in plant-fungus interactions was evaluated. C. herbarum pGM is composed of carbohydrates (76%) and contains mannose, galactose and glucose as its main monosaccharides (molar ratio, 52:36:12). Methylation and C-13-nuclear magnetic resonance (C-13-NMR) spectroscopy analysis have shown the presence of a main chain containing (1 - 6)-linked a-D-Manp residues, and beta-D-Galf residues are present as (1 - 5)-interlinked side chains. beta-Galactofuranose containing similar structures were characterized by our group in A. fumigants, A. versicolor, A. flavus and C. resinae.