Phyllosphere of three medicinal plants viz., Calotropis procera, Azadirachta indica and Datura stramonium from Bikaner region was analyzed. Microflora was analyzed by leaf impression method and serial dilution method. A range of bacteria and fungi were isolated and identified by colony characters, staining and biochemical tests on the basis of Bergey's manual. Microbial diversity from Datura stramonium included Micrococcus leuteus, Bacillus subtilis, Bacillus polymyxa and Fusarium oxysporum. While from phylloplane of Calotropis procera two bacterial species (Bacilus subtilis and Escherichia colt) and two fungal, species (Fusarium oxysporum and Aspergillus niger) were identified. Microflora of Azadirachta indica was found to have Bacillus subtilis, Corynebacterium xerosis, Corynebacterium bovis and Aspergillus niger. These observations will help us to understand the behavior and interactions of epiphytic microbes with plants as well as among themselves.
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