Effect of Different Plant Growth Regulators on Plant Regeneration from Hypocotyl Explants of Lycopersicon esculentum Mill.cv. Pusa Ruby

摘要

Hypocotyl explants cultured on M.S. medium supplemented with different IAA (1.0 mg/1), NAA (1.0 mg/1), 2, 4-D (1.0 mg/1) alone produced white callus. Whereas callusing was more pronounced in 1.0 mg/1 IAA, compared to NAA (1.0 mg/1) and 2,4-D (1.0 mg/1). Hypocotyl explants cultured on M.S. medium supplemented with BAP (1.0 mg/1) or Kn (1.0 mg/1) alone showed better response in shoot differentiation with green callus. However, BAP (2.0 mg/1) showed good results compared to Kinetin (2.0 mg/1) in inducing shoot buds. IAA (1.0 mg/1) + BAP (2.0 mg/1) and IAA (1.0 mg/1) + Kn (2.0 mg/1) gave good response and produced shoots along with green nodular callus with more number of shoots buds. The combination of IAA (1.0 mg/1) .+ BAP (5.0 mg/1) found to be better medium for more number of shoots induction compared to all other concentrations of IAA + Kn. Maximum number of shoots was observed in the combination of IAA 1.0 mg/1 + BAP 5.0 mg/1. Thus the plant regeneration was established on MS +1.0 mg/1 IAA + 2.0mg/1 BAP in tomato cv pusa ruby from hypocotyls explants, successfully.