Pro-apoptotic activity of acylated triterpenoid saponins from the stem bark of Albizia chevalieri harms

摘要

Graphical abstractDisplay OmittedHighlights?Three new acylated glycosides of echinocystic acid, named chevalierosides A–C (1–3) were isolated from the stem bark ofAlbizia chevalieri.?Their structures were established on the basis of extensive analysis of 1D and 2D NMR experiments.?Cytometric analyses showed that the isolated saponins induced apoptosis of two human cell lines (AsPC-1 and THP-1) in a dose-dependent manner.As a continuation of our interest in apoptosis-inducing triterpenoid saponins fromAlbiziagenus, phytochemical investigation of the stem bark ofAlbizia chevalieriled to the isolation of three new oleanane-type saponins, named chevalierosides A–C (1–3). Their structures were established on the basis of extensive analysis of 1D and 2D NMR (1H-,13C NMR, DEPT, COSY, TOCSY, ROESY, HSQC and HMBC) experiments, HRESIMS studies, and by chemical evidence. The pro-apoptotic effect of the three saponins was evaluated on two human cell lines (pancreatic carcinoma AsPC-1 and hematopoietic monocytic THP-1). Cytometric analyses showed that saponins1–3induced apoptosis of both human cell lines (AsPC-1 and THP-1) in a dose-dependent manner.