Chlorpyrifos Induced Dermal Toxicity in Albino Rabbits

摘要

Organophosphates being acetylcholine esterase inhibitors are immoderately used as insecticides on a variety of agriculture crops. Toxicity of such compounds like chlorpyrifos (CPF) poses serious health issues to the individuals at risk. The concentrations below the probable occupational exposure were selected to ascertain the extent of dermal damage in experimentally exposed animals. To determine CPF induced dermal toxicity, a total of 24 adult albino rabbits of both genders and of the same age group was categorized as group A and B, respectively. Group A was further sub grouped into A1 (all males) and A2 (all females). Similarly, group B was divided into B1 (all males) and B2 (all females). Four different dosing sites of one square inch each were selected. For naked topical CPF application, each animal of sub group A1 and A2 topically dosed 500 mu l of 20%, 30% CPF ethanolic solution, its commercial formulation and ethanol (control) on four dosing sites separately. The dosage of CPF solutions (20% & 30%), commercial formulation and ethanol (control) were repeated every 24 hours for 72 hours and observed for 96 hours. Similar groups were made for occluded topical CPF. Treated animals were observed for gross dermal lesions (erythema scoring of varying grades). Skin tissues from treated sites were taken and processed for histopathological and morphometric changes. Erythema scoring was increased in animals treated with high doses of CPF both in different concentration and commercial preparations in a dose dependent response. The number of layers of erythema in chemically exposed skins of rabbits was also increased in dose dependent response both in naked and occluded groups. The dermal insult induced in both exposure concentrations at par with the commercial preparation indicated the level of toxicity of this compound and public health consequences might be predicted. (C) 2018 PVJ. All rights reserved