An effective method for extracting total RNA from young embryo of seedless litchi.

摘要

A total RNA extraction method for young embryo of seedless litchi was introduced. CTAB, Phenol (saturated with water), chloroform, Guanidine isothiocyanate were used as main extraction reagents. Polyphenolic compounds were removed effectively by added PVP into the extraction buffer solution. RNA was purified intensively by phenol, chloroform extraction, and ethanol deposition after deposited by LiCl. Both the results of formaldehyde denatured agarose gel electrophoresis and ultraviolet spectrophotometer analysis showed high integrity and purity of RNA. So the quality of extracted RNA could meet the demand of most molecular biology experiments that require higher quality RNA.