Sequential Coalescence Enabled Two‐Step Microreactions in Triple‐Core Double‐Emulsion Droplets Triggered by an Electric Field

Jia Yankai; Ren Yukun; Hou Likai; Liu Weiyu; Deng Xiaokang; Jiang Hongyuan

摘要

Abstract >Advances in microfluidic emulsification have enabled the generation of exquisite multiple‐core droplets, which are promising structures to accommodate microreactions. An essential requirement for conducting reactions is the sequential coalescence of the multiple cores encapsulated within these droplets, therefore, mixing the reagents together in a controlled sequence. Here, a microfluidic approach is reported for the conduction of two‐step microreactions by electrically fusing three cores inside double‐emulsion droplets. Using a microcapillary glass device, monodisperse water‐in‐oil‐in‐water droplets are fabricated with three compartmented reagents encapsulated inside. An AC electric field is then applied through a polydimethylsiloxane chip to trigger the sequential mixing of the reagents, where the precise sequence is guaranteed by the discrepancy of the volume or conductivity of the inner cores. A two‐step reaction in each droplet is ensured by two times of core coalescence, which totally takes 20–40 s depending on varying conditions. The optimal parameters of the AC signal for the sequential fusion of the inner droplets are identified. Moreover, the capability of this technique is demonstrated by conducting an enzyme‐catalyzed reaction used for glucose detection with the double‐emulsion droplets. This technique should benefit a wide range of applications that require multistep reactions in micrometer scale. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >前进微流体乳化使得能够产生精致的多芯液滴，这是容纳微孔的结构。导电反应的基本要求是封装在这些液滴内的多芯的连续聚结，因此，将试剂混合在一起以受控序列混合在一起。这里，据报道，通过在双乳液液滴内电熔化三个芯来传导两步微孔的微流体方法。使用微毛细管玻璃装置，用内部封装的三个隔室试剂制造单分散水包内液滴。然后通过聚二甲基硅氧烷芯片施加AC电场以触发试剂的顺序混合，其中通过内芯的体积或电导率的差异来保证精确序列。通过两次核心聚结的两倍于每滴进行两步反应，这取决于不同条件，完全需要20-40秒。识别用于内液滴的顺序融合的AC信号的最佳参数。此外，通过用双乳液液滴进行用于葡萄糖检测的酶催化反应来证明该技术的能力。这种技术应该有益于需要在千分尺刻度中的多步骤反应的广泛应用。</ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-28247/'>《Small》</a> <b style="margin: 0 2px;">|</b><span>2017年第46期</span><b style="margin: 0 2px;">|</b><span>共1页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jia Yankai&option=202" target="_blank" rel="nofollow">Jia Yankai;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ren Yukun&option=202" target="_blank" rel="nofollow">Ren Yukun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hou Likai&option=202" target="_blank" rel="nofollow">Hou Likai;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Weiyu&option=202" target="_blank" rel="nofollow">Liu Weiyu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Deng Xiaokang&option=202" target="_blank" rel="nofollow">Deng Xiaokang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jiang Hongyuan&option=202" target="_blank" rel="nofollow">Jiang Hongyuan;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of Mechatronics EngineeringHarbin Institute of TechnologyHarbin 150001 China;</p> <p>School of Mechatronics EngineeringHarbin Institute of TechnologyHarbin 150001 China;</p> <p>School of Mechatronics EngineeringHarbin Institute of TechnologyHarbin 150001 China;</p> <p>School of Mechatronics EngineeringHarbin Institute of TechnologyHarbin 150001 China;</p> <p>School of Mechatronics EngineeringHarbin Institute of TechnologyHarbin 150001 China;</p> <p>School of Mechatronics EngineeringHarbin Institute of TechnologyHarbin 150001 China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/6960.html" title="特种结构材料">特种结构材料;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=double emulsions&option=203" rel="nofollow">double emulsions;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=droplets&option=203" rel="nofollow">droplets;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=electrocoalescence&option=203" rel="nofollow">electrocoalescence;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microfluidics&option=203" rel="nofollow">microfluidics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=two‐step microreactions&option=203" rel="nofollow">two‐step microreactions;</a> </p> <div class="translation"> 机译：双乳液;液滴;电晕;微流体;两步微孔; 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<a href="/journal-cn-7432/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 浙江大学学报（工学版） </a> </span> <span> . 2021</span><span>,第007期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-1005_thesis/0202242934.html">双液滴在交流电场下的变形和相互作用</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈晓东&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈晓东</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=胡国庆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,胡国庆</a> <span> <a href="/conference-cn-1005/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 北京力学会第21届学术年会暨北京振动工程学会第22届学术年会 </a> <span> <span> . 2015</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315874379.html">高压电场下W/O乳状液液滴-液滴、液滴-界面聚并行为研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=任瑞娟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 任瑞娟</a> <span> . 2018</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120111726147.html">用于促进油连续相乳液中的液滴聚结的方法和装置</a> <b>[P]</b> . <span> 中国专利： CN111683726A </span> <span> . 2020-09-18</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061203449847.html">一种电场与剪切场耦合作用下双液滴聚结的微观实验装置</a> <b>[P]</b> . <span> 中国专利： CN107238564B </span> <span> . 2019.08.20</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130445855259.html">Moistening and inking process consists of first step of forming emulsion from printing ink and moistener and second step of dosing emulsion in jet or droplet form</a> <b>[P]</b> . <span> 外国专利：  DE10212537A1 </span> <span> . 2002-10-31</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：润湿和着墨过程包括第一步，由印刷油墨和润湿剂形成乳液，第二步以喷射或液滴形式投加乳液 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130444193912.html">Electric field enhanced coalescence of silicone emulsions</a> <b>[P]</b> . <span> 外国专利：  EP0841362B1 </span> <span> . 2003-06-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：电场增强聚硅氧烷乳液的聚结 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130452192526.html">Electric field enhanced coalescence of silicone emulsions</a> <b>[P]</b> . <span> 外国专利：  EP0841362A3 </span> <span> . 1999-01-07</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: 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