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Interactions of Modern Aesthetic Materials for Prosthetic Restorations with Oral Mucosa A pilot study

机译:现代美学材料与口腔粘膜假肢修复的相互作用试验研究

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摘要

The specific objective of the present study is to assess the interactions between cells from a human gingival epithelial cell line and various aesthetic materials used in modern prosthetic dentistry. For this study six types of dental materials were selected: Cr-Co non-precious metal alloy, ceramics applied on Cr-Co nonprecious metal alloy, zirconia, ceramics applied on zirconia, polymethyl methacrylate and pressed ceramics/ lithium disilicate. Cells from a human gingival epithelial cell line, Ca9-22 (Health Science Research Resources Bank), were cultured on the chosen surfaces for 3, 5 and 7 days. Cellular proliferation, cell attachment (using Multiplex Arrays Technology) and cytotoxicity (MTT-Assay) were evaluated at distinct predetermined intervals. Measurements performed at each distinct predetermined interval showed no significant difference for cell proliferation and cytotoxicity between the selected surfaces, however the highest levels were registered for the polymethyl methacrylate surface. Different attachment patterns were observed for epithelial cells attached on substrates, such as significantly different levels of adherence of E-Cadherin and N-Cadherin molecules; E-Cadherin adhesion levels indicate that pressed ceramics may be the dental material which, compared to the selected materials, influences the least the homeostasis of oral mucosa.
机译:本研究的具体目的是评估来自人牙龈上皮细胞系和现代假肢牙科的各种美学材料之间的细胞之间的相互作用。本研究选择了六种类型的牙科材料:Cr-Co非贵金属合金,应用于Cr-Co非匮乏金属合金,氧化锆,涂覆在氧化锆上的陶瓷,聚甲基丙烯酸甲酯和压制陶瓷/锂静止。来自人牙龈上皮细胞系CA9-22(卫生科学研究资源库)的细胞在选定的表面上培养3,5和7天。细胞增殖,细胞附着(使用多重阵列技术)和以不同的预定间隔评价细胞毒性(MTT-测定)。在每个不同的预定间隔下进行的测量对于所选表面之间的细胞增殖和细胞毒性没有显着差异,但是对于聚甲基丙烯酸甲酯表面注册了最高水平。对于附着在基材上的上皮细胞,观察到不同的附着图案,例如电子钙粘蛋白和N-Cadherin分子的显着不同的粘附程度; E-钙粘蛋白的粘附水平表明,压制陶瓷可以是与所选材料相比的牙科材料,影响口腔粘膜的最小稳态。

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