Concomitant Quantification of Caffeine, Cotinine and N-methyl Uric Acid in Urine Applications for athlete monitoring and pharmacological screening

摘要

Simultaneous determination of caffeine, cotinine, and 1N-methyluric acid in urine has numerous applications in determining patterns of use by athletes, according to the World Anti-Doping Agency (WADA) which includes these substances on the Monitoring List. The method can provide information on the enzyme-inducing activity on CYP1A2 of polycyclic aromatic hydrocarbons from cigarette smoke. Urine samples from 30 people (15 smokers and 15 non-smokers) were sampled 6 hours after having consumed a beverage with a total caffeine content of 200 mg. A HPLC with UV detection method was used which allowed concomitant determination of the three analytes. The results obtained show different values of caffeine urine concentrations (6.47 +/- 3.63 mu M/L in smokers vs 10.09 +/- 5.68 in non-smokers, p 0.05) and a higher elimination of N1-methyluric acid by over 50% in smokers (identifiable due to the presence of cotinine, main metabolite of nicotine).