A case of non-monotonous influence of pressure on enantioselectivity in supercritical fluid chromatography

摘要

non-monotonous dependence of selectivity coefficient on pressure in the enantioseparation of salbutamol sulfate by supercritical fluid chromatography was discovered. When increasing the isothermal pressure of the mobile phase in the interval 100-200 bar at a temperature of 20 degrees C, the selectivity coefficient of separation of salbutamol enantiomers first increased, then decreased, followed by another increase. Such a behavior is atypical and determines the expediency of using pressure to control selectivity in this kind of chromatography.