Radicals initiated by gamma-rays in collagen and its main components

摘要

Radical products generated by gamma-rays were identified in collagen and in microcrystalline powders of glycyne, L-proline and L-hydroksyproline. Reagents irradiated at 77 K were studied by EPR spectroscopy in the range of 100-350 K using temperature control system. Two radical centers found in collagen were localized in proline ring at a and gamma positions to the carbonyl group. There were neither terminal radicals generated by chain scission nor transient products disrupting hydrogen bonding system of irradiated collagen.