GC-MS and FT-IR Analyses of Phytocomponents From petroleum ether Fraction of Leaf Extract of Psidium guajava

摘要

Background and Objectives: Phytocomponents from plant materials are largely responsible for their biologic activities. The phytocomponents from petroleum ether fraction of the leaf extract of P. guajava was identified, quantified and characterized. Materials and Methods: Chromatographic and spectrophotometric methods, namely, Gas chromatography-mass spectrometry (GC-MS) and Fourier transform-infrared spectrometry (FT-IR) were used for the identification, quantification and characterization of the phytocomponents. Results: The major phytocomponent from petroleum ether fraction of the leaf extract of P. guajava was bis (2-ethylhexyl) phthalate, whereas the minor phytocomponents were 2-pentadecanol and 14-pentadecenoic acid. Petroleum ether fraction of leaf extract of P. guajava gave a characteristic broad peak around 3328.5 cm~(-1), which was indicative of the presence of an alcohol functional group. Conclusion: GC-MS and FT-IR analyses of petroleum ether fraction of leaf extract of P. guajava identified five phytocomponents viz., 2-pentadecanol, carbonic acid, eicosyl vinyl ester, bis (2-ethylhexyl) phthalate, 14-pentadecenoic acid and 2-methyltetracosane.