Vitrification negatively affects the Ca2+-releasing and activation potential of mouse oocytes, but vitrified oocytes are potentially useful for diagnostic purposes

Bonte Davina; Thys Vanessa; De Sutter Petra; Boel Annekatrien; Leybaert Luc; Heindryckx Bjoern

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Vitrification negatively affects the Ca2+-releasing and activation potential of mouse oocytes, but vitrified oocytes are potentially useful for diagnostic purposes

机译：玻璃化对小鼠卵母细胞的Ca2 + -Releleating和活化电位产生负面影响，但玻璃化卵母细胞可能用于诊断目的

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Research question: To what extent does vitrification affect the Ca2+-releasing and activation potential of mouse oocytes, which are commonly used to determine the oocyte activation potential of human spermatozoa?

机译：研究问题：玻璃化程度如何影响小鼠卵母细胞的Ca2 +咯咯和激活潜力，这通常用于确定人体精子的卵母细胞活化潜力吗？

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来源
《Reproductive biomedicine online 》 |2020年第1期| 共13页
作者
Bonte Davina; Thys Vanessa; De Sutter Petra; Boel Annekatrien; Leybaert Luc; Heindryckx Bjoern;
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作者单位

Ghent Univ Hosp Dept Human Struct &

Repair Ghent Fertil &

Stem Cell Team G FaST C Heymanslaan 10;

Ghent Univ Hosp Dept Human Struct &

Repair Ghent Fertil &

Stem Cell Team G FaST C Heymanslaan 10;

Ghent Univ Hosp Dept Human Struct &

Repair Ghent Fertil &

Stem Cell Team G FaST C Heymanslaan 10;

Ghent Univ Hosp Dept Human Struct &

Repair Ghent Fertil &

Stem Cell Team G FaST C Heymanslaan 10;

Univ Ghent Dept Basic &

Appl Med Sci Physiol Grp C Heymanslaagn 10 B-9000 Ghent Belgium;

Ghent Univ Hosp Dept Human Struct &

Repair Ghent Fertil &

Stem Cell Team G FaST C Heymanslaan 10;

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原文格式 PDF
正文语种 eng
中图分类人体生理学 ;
关键词
Assisted oocyte activation; Calcium; Cryopreservation; Diagnostic test; ICSI; Vitrification;

机译：辅助卵母细胞活化;钙;冷冻保存;诊断测试;ICSI;玻璃化;

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1. Vitrification negatively affects the Ca2+-releasing and activation potential of mouse oocytes, but vitrified oocytes are potentially useful for diagnostic purposes [J] . Bonte Davina, Thys Vanessa, De Sutter Petra, Reproductive biomedicine online . 2020 ,第1期

机译：玻璃化对小鼠卵母细胞的Ca2 + -Releleating和活化电位产生负面影响，但玻璃化卵母细胞可能用于诊断目的
2. Prematuration Culture with Phosphodiesterase Inhibitors After Vitrification May Induce Recovery of Mitochondrial Activity in Vitrified Mouse Immature Oocytes [J] . Yoon Sook Young, Eum Jin Hee, Cha Soo Kyoung, Biopreservation and biobanking . 2018 ,第4期

机译：在玻璃化后，具有磷酸二酯酶抑制剂的前饱和培养物可诱导玻璃化小鼠未成熟卵母细胞中的线粒体活性恢复
3. Prematuration Culture with Phosphodiesterase Inhibitors After Vitrification May Induce Recovery of Mitochondrial Activity in Vitrified Mouse Immature Oocytes [J] . Yoon Sook Young, Eum Jin Hee, Cha Soo Kyoung, Biopreservation and biobanking . 2018 ,第4期

机译：在玻璃化后，具有磷酸二酯酶抑制剂的前置培养物可以诱导玻璃化小鼠未成熟卵母细胞中的线粒体活性恢复
4. Comparison of sucrose and trehalose media modification as an update of oocyte vitrification: A study of apoptotic level [C] . Silvia W. Lestari, Nurin N. Fitriyah, Mulyoto Pangestu, International Symposium of Biomedical Engineering . 2018

机译：蔗糖和海藻糖介质改性的比较作为卵母玻璃质的更新：凋亡水平的研究
5. Embryonic development of in vitro matured mouse oocytes following vitrification and in vitro fertilization. [D] . Yu, Xiaomin. 2011

机译：玻璃化和体外受精后，体外成熟小鼠卵母细胞的胚胎发育。
6. Development of Optimized Vitrification Procedures Using Closed Carrier System to Improve the Survival and Developmental Competence of Vitrified Mouse Oocytes [O] . Jae Kyun Park, Ju Hee Lee, Eun A Park, 2021

机译：使用封闭式载体系统的优化玻璃化程序的开发提高玻璃卵卵卵母细胞的存活率和发育能力
7. The survival of mouse oocytes shows little or no correlation with the vitrification or freezing of the external medium, but the ability of the medium to vitrify is affected by its solute concentration and by the cooling rate [O] . Estefania Paredes, Peter Mazur 2013

机译：小鼠卵母细胞的存活表现出与外部介质的玻璃化或冷冻的少量或无相关性，但培养基玻璃化的能力受其溶质浓度和冷却速率的影响

Vitrification negatively affects the Ca2+-releasing and activation potential of mouse oocytes, but vitrified oocytes are potentially useful for diagnostic purposes

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