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A rapid sample preparation method for the analysis of stable isotope ratios of beef samples from different countries

机译：一种快速的样品制备方法，用于分析不同国家牛肉样本稳定同位素比

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Rationale >The use of multi‐isotopic analyses to trace beef is gaining wider acceptance, but no uniform standard for the pretreatment method is available for the determination of stable isotope ratios. Drying and defatting of meat samples are usually applied. Thus, a rapid sample preparation procedure is required to provide a reference for the study of beef using stable isotope methods. </section> <section xml:id="rcm8795-sec-0002" numbered="no"> <title type="main">Methods > Student's t ‐test ( T ‐test) was used to determine significant differences between the δ 13 C and δ 15 N values in traditional and rapid beef sample preparation methods. The δ 13 C, δ 15 N, δ 2 H, and δ 18 O values of beef samples from six countries were assayed using elemental analyzer–isotope ratio mass spectrometry. Stable isotope data were subjected to principal component analysis, discriminant analysis, and partial least‐squares discriminant analysis (PLS‐DA). </section> <section xml:id="rcm8795-sec-0003" numbered="no"> <title type="main">Results > There was no significant difference ( P ??0.05) between the δ 13 C and δ 15 N values of the two preparation approaches. A classification of satisfactory was obtained with the original‐validation rate of 96.6% and the cross‐validation rate of 95.9%. The PLS‐DA model was correctly validated to differentiate beef samples from six countries. </section> <section xml:id="rcm8795-sec-0004" numbered="no"> <title type="main">Conclusions >We describe a rapid sample preparation method for beef samples. A model combining stable isotope data and chemometric methods correctly assigned the origin of beef samples from different countries. The results demonstrated the successful utilization of rapid pretreatment methods to prepare beef samples when using multiple stable isotope analyses to trace beef samples from different countries. </section> </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <第XML：ID =“RCM8795-SEC-0001”编号=“否”> <标题类型=“main”>基本原理</ title> >使用多同位素分析以追踪牛肉是更广泛的验收，但没有用于预处理方法的均匀标准可用于测定稳定同位素比率。通常施加干燥和肉类样品的脱落。因此，需要快速样品制备方法来提供使用稳定的同位素方法研究牛肉的研究。</ p> </部分> <第XML：ID =“RCM8795-SEC-0002”编号=“否”> <标题类型=“main”>方法</ title> > 学生们 t </ i> -测试（ t </ i> - 最低）用于确定差异之间的显着差异 δ</ i> 13 </ sup> C和 δ</ i> 15 </ sup> N个传统和快速牛肉样品制备方法的值。这 δ</ i> 13 </ sup> C， δ</ i> 15 </ sup> n， δ</ i> 2 </ sup> 手 δ</ i> 18 </ sup> 使用元素分析仪同位素比率质谱法测定来自六个国家的牛肉样本的值。对稳定的同位素数据进行主成分分析，判别分析和局部最小二乘判别分析（PLS-DA）。 </ p> </部分> <第XML：ID =“RCM8795-SEC-0003”编号=“否”> <标题类型=“main”>结果</ title> > 没有显着差异（ p </ i> ？＆？0.05） δ</ i> 13 </ sup> C和 δ</ i> 15 </ sup> 两种准备方法的n值。获得令人满意的分类，原始验证率为96.6％，交叉验证率为95.9％。 PLS-DA模型被正确验证以区分六个国家的牛肉样本。 </ p> </部分> <第XML：ID =“RCM8795-SEC-0004”编号=“否”> <标题类型=“main”>结论</ title> 我们描述了一种牛肉样品的快速样品制备方法。组合稳定同位素数据和化学计量方法的模型正确地分配了不同国家的牛肉样本的起源。结果表明，在使用多个稳定同位素分析到来自不同国家的痕量牛肉样品时，成功利用快速预处理方法来制备牛肉样品。</ p> </部分> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-26627/'>《Rapid Communications in Mass Spectrometry: RCM》</a> <b style="margin: 0 2px;">|</b><span>2020年第13期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> </p> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704019740928.html">A rapid sample preparation method for the analysis of stable isotope ratios of beef samples from different countries</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-21493/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Journal of Modern Physics, B. 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href="/patent-detail/061204478283.html">通过引入系列不同同位素标签用于快速实时定量样品中目标分析物的检测方法</a> <b>[P]</b> . <span> 中国专利： CN112730710B </span> <span> . 2021.06.22</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120112687806.html">通过引入系列不同同位素标签用于快速实时定量样品中目标分析物的检测方法</a> <b>[P]</b> . <span> 中国专利： CN112730710A </span> <span> . 2021-04-30</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130400125725.html">Stable isotope ratio analysis sample collection method, stable isotope ratio analysis method, production area discrimination method, and stable isotope ratio analysis sample collection device</a> <b>[P]</b> . <span> 外国专利：  JP6715088B2 </span> <span> . 2020.07.01</span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130407668603.html">STABLE ISOTOPE RATIO ANALYSIS SAMPLE COLLECTION METHOD, STABLE ISOTOPE RATIO ANALYSIS METHOD, GEOGRAPHIC ORIGIN IDENTIFICATION METHOD, AND STABLE ISOTOPE RATIO ANALYSIS SAMPLE COLLECTION DEVICE</a> <b>[P]</b> . <span> 外国专利：  JP2017215212A </span> <span> . 2017-12-07</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：稳定同位素比分析样品收集方法，稳定同位素比分析方法，地理原点识别方法和稳定同位素比分析样品收集设备 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130424572240.html">METHOD OF ANALYZING THE ISOTOPE OF LITHIUM, CAPABLE OF CONVENIENTLY AND RAPIDLY PERFORMING ALL PROCEDURES COMPRISING THE PRE-PROCESSING OF SAMPLE, THE SEPARATION AND RECOVERY OF LITHIUM AND THE ANALYSIS OF THE ISOTOPE OF LITHIUM</a> <b>[P]</b> . <span> 外国专利：  KR20110046965A </span> <span> . 2011-05-06</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：分析锂同位素的方法，能够方便，快速地执行包括样品预处理，锂的分离和回收以及锂同位素分析的所有过程 </span> </p> 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