Isolation and identification of bioactive substance 1-hydroxyphenazine fromPseudomonas aeruginosaand its antimicrobial activity

摘要

A strain named asPseudomonas aeruginosa2016NX1, which could produce phenazine and cereusitin, was isolated from the root ofMillettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purified materials were identified by analysis of nuclear magnetic resonance. The major yellow component is 1-hydroxyphenazine and the minor blue component is cereusitin A. The tests of antimicrobial activity of yellow component showed that the growth of several common plant pathogenic fungi and bacteria (such asCochliobolus miyabeanus,Diaporthe citri,Salmonellasp.,Klebsiella oxytoca) could be strongly inhibited. This study suggested thatPseudomonas aeruginosastrain 2016NX1 had a significant potential for biological control of phytopathogenic fungi.