Synthesis, crystallization and preliminary crystallographic analysis of a 52-nucleotide DNA/2-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with a substrate

摘要

A 52-nucleotide DNA/2-OMe-RNA oligomer mimicking 10-23 DNAzyme in the complex with its substrate was synthesized, purified and crystallized by the hanging-drop method using 0.8M sodium potassium tartrate as a precipitant. A data set to 1.21 angstrom resolution was collected from a monocrystal at 100K using synchrotron radiation on a beamline BL14.1 at BESSY. The crystal belonged to the P2(1) group with unit-cell a = 49.42, b = 24.69, c = 50.23, = 118.48.