Evaluation of Genetic Diversity in Rice (Oryza sativa L.) Using Simple Sequence Repeats (SSR) Markers

摘要

In the study, 10 rice (Oryza sativa L.) accessions comprising of biofortified, aerobic, japonica donors, checks represented by indica erstwhile mega-varieties and traditional accessions differing in drought tolerance were screened for genetic diversity. These biofortified lines are a product of intense breeding efforts. Out of the 45 SSR markers used, 12 SSR markers were highly informative and polymorphic. A total of 36 alleles were detected with an average of three alleles per locus. The polymorphicinformation content values ranged from 0.325 (RM315) to 0.791 (RM260) in all the 12 loci with an average of 0.494. RM 260 found as the best marker for identification of genotypes as revealed by PIC values. The informative and highly informative markersidentified in the study could be utilized in further studies for association mapping and marker assisted selection for drought tolerance in rice genotypes. The studied genotypes revealed high level of genetic diversity indicating the availability of suchmaterials for rice breeding program for drought tolerance.