Enzymatic Lysis of Living Microbial Cells: A Universal Approach to Calculating the Rate of Cell Lysis in Turbidimetric Measurements

摘要

The data of the turbidimetric measurement for the enzymatic lysis of various living bacterial cells are analyzed. A method for the correct recalculation of the turbidimetric data (–Δ A /Δ t ) into absolute values of the change in the concentration of living cells (–ΔCFU/Δ t ) is proposed. The dimensionless efficiency of cell lysis–(1/CFU~(0)) · ΔCFU/Δ t for various bacterial cells is calculated to correctly compare the efficiency of the action of different bacteriolytic factors on various bacterial cells.