The Chinese Herbs Scutellaria baicalensis and Fritillaria cirrhosa Target NF kappa B to Inhibit Proliferation of Ovarian and Endometrial Cancer Cells

摘要

The herbs Scutellaria baicalensis (SB) and Fritillaria cirrhosa (FC) are widely used in Chinese medicine to treat several aliments and as an adjuvant to chemotherapy of lung cancer. No information is available regarding the two herbs' influence on ovarian and endometrial cancer. To fill this data gap we compared cell growth responses to SB and FC in ovarian and endometrial cancer cell lines. Dose-dependent cell growth inhibition was observed following higher doses in all cell lines while lower doses stimulated growth in only endometrial cell lines. Higher doses of SB and FC significantly decreased cell growth on soft agar and decreased the invasive potential of cancer cells. Treatment of cells with both herbs resulted in activation of caspase-3, G(0)/G(1) phase cell cycle arrest, downregulation of cyclins D1 and D3 and induction of p27. Both herbs decreased NFB DNA binding, reduced expression of phosphorylated IB, abrogated NFB activation, and downregulated NFB-regulated metastasis-promoting proteins in cancer cells. Furthermore, knockdown of NFB attenuated SB- and FC-induced cell growth inhibition. These results suggest that inhibition of NFB activation may be an important mechanism for growth suppression by SB and FC. Data indicate that these herbs may represent a new source of agents for NFB inhibition in cancer therapy. (c) 2013 Wiley Periodicals, Inc.