Polyclonal antibodies to Paracoccidioides brasiliensis Paracoccidioides brasiliensis are able to recognise antigens from different strains from Paracoccidioides Paracoccidioides species complex, including Paracoccidioides lutzii Paracoccidioides lutzii LDR LDR 2

摘要

Summary Since the new species Paracoccidioides lutzii emerged in 2009, much has been researched on strains previously considered atypical. Still, there is no consensus about recognition of antigens from P.?lutzii by antibodies directed to other Paracoccidioides species, which can have great impact on Paracoccidioidomycosis ( PCM ) diagnosis. Current research investigated soluble protein/carbohydrate epitopes from P.?lutzii LDR 2, Paracoccidioides?restrepiensis B339 and Paracoccidioides?americana LDR 3 recognised by IgG directed to Paracoccidioides?brasiliensis . Cell free antigens ( CFA ) were analysed by: (a)silver and periodic acid‐Schiff staining of SDS ‐ PAGE ; (b)immunoblot ( IB ) with rabbit IgG anti‐ P.?brasiliensis Pb18; (c) IB and ELISA with a pool of PCM patients’ sera before and after treatment with sodium metaperiodate ( SMP ) to oxidise carbohydrate epitopes. Both rabbit and human immune sera recognised several antigens of P.?lutzii LDR 2, P.?restrepiensis B339 and P.?americana LDR 3. P.?lutzii 's gp43 was not observed in IB or silver/ PAS staining. SMP treatment affected reactions with all 3 CFA s, but more intensely with antigens from P.?lutzii LDR 2. In conclusion, antibodies directed to P.?brasiliensis recognised antigens from P.?lutzii LDR 2. The use of any of the recognised antigens in a broad spectrum diagnostic model for Paracoccidioides species complex needs to be further investigated.